Thursday, February 4, 2010

True or False. Restriction enzymes cut both strands of a double stranded DNA Molecule Please explain??

True. The enzyme makes cuts in the backbone of both strands.





Commonly used restriction enzymes bind DNA as dimers--two identical subunits, each binding to one side of the DNA, and each makes its own cut. The sequence of DNA recognized by this type are called palindromic--they read the same on each side of the DNA. Here is an example:





GGATCC


CCATGG





The top strand reads the same as the bottom strand backwards, so there is an identical sequence for each individual side.True or False. Restriction enzymes cut both strands of a double stranded DNA Molecule Please explain??
True, the endonuclease binds around the DNA and clips both strands. Some enzymes leave blunt ends (SmaI) and some enzymes leave sticky ends (EcoRI) but in all cases both strands are cut.

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